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验证数据展示

  • FC experiment of mouse splenocytes using CL488-98025

    FC experiment of mouse splenocytes using CL488-98025

    1x10^6 unstimulated (left) or LPS treated (right) mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL488-98025, Clone:230476B8). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL488-98025

    FC experiment of mouse splenocytes using CL488-98025

    1x10^6 LPS treated mouse splenocytes were surface stained with 0.25ug CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL488-98025, Clone: 230476B8) (red) or Isotype Control (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody (CL488-98025, Clone: 230476B8) (black, dashed). Cells were not fixed.

  • IF Staining of RAW 264.7 using CL488-98025

    IF Staining of RAW 264.7 using CL488-98025

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CoraLite® Plus 488 CD86 antibody (CL488-98025, Clone: 230476B8 ) at dilution of 1:200.

CoraLite® Plus 488 Anti-Mouse CD86 Rabbit Recombinant Antibody

CD86 Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. CL488-98025

产品说明书

CloneNo. 230476B8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IF/ICC, FC

别名

230476B8, B7, B7 2, B7.2, B70

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells
Positive FC detected inLPS treated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98025-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-98025 targets CD86 in IF/ICC, FC applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 mouse
免疫原 CD86 fusion protein Eg0802 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD86 antigen
别名 230476B8, B7, B7 2, B7.2, B70
计算分子量35 kDa
GenBank蛋白编号NM_019388
基因名称 Cd86
Gene ID (NCBI) 12524
RRIDAB_3673370
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42082-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD86 antibody CL488-98025Download protocol
FC protocol for CL Plus 488 CD86 antibody CL488-98025Download protocol
Standard Protocols
Click here to view our Standard Protocols