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验证数据展示

  • WB analysis using 83523-4-RR (same clone as 83523-4-PBS)

    WB analysis using 83523-4-RR (same clone as 83523-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83523-4-RR (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 83523-4-RR (same clone as 83523-4-PBS)

    IF Staining of Raji using 83523-4-RR (same clone as 83523-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD86 antibody (83523-4-RR, Clone: 240429G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83523-4-RR

    Affinity and Kinetic Characterization of 83523-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83523-4-RR against Human CD86 were performed. The affinity constant is 0.766 nM.

CD86 Recombinant monoclonal antibody, PBS Only

CD86 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83523-4-PBS

产品说明书

CloneNo. 240429G12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

240429G12, Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83523-4-PBS targets CD86 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0966

Product name: Recombinant Human CD86 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-247 aa of NM_175862

Sequence: LKIQAYFNETADLPCQFANSQNQSLSELVVFWQDQENLVLNEVYLGKEKFDSVHSKYMGRTSFDSDSWTLRLHNLQIKDKGLYQCIIHHKKPTGMIRIHQMNSELSVLANFSQPEIVPISNITENVYINLTCSSIHGYPEPKKMSVLLRTKNSTIEYDGVMQKSQDNVTELYDVSISLSVSFPDVTSNMTIFCILETDKTRLLSSPFSIELEDPQPPPDHIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD86 molecule
别名 240429G12, Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70
计算分子量 329 aa, 38 kDa
观测分子量70 kDa
GenBank蛋白编号NM_175862
基因名称 CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP42081
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

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