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验证数据展示

  • FC experiment of mouse splenocytes using 98076-1-RR

    FC experiment of mouse splenocytes using 98076-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD84 Rabbit RecAb (98076-1-RR, Clone: 240903B8) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed.

  • FC experiment of mouse splenocytes using 98076-1-RR

    FC experiment of mouse splenocytes using 98076-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD84 Rabbit RecAb (98076-1-RR, Clone: 240903B8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98076-1-RR

    Affinity and Kinetic Characterization of 98076-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98076-1-RR against Mouse CD84/SLAMF5 were performed. The affinity constant is 0.297 nM.

Anti-Mouse CD84 Rabbit Recombinant Antibody

CD84 Uni-rAb® Recombinant Antibody for FC
Cat No. 98076-1-RR

产品说明书

CloneNo. 240903B8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD84/SLAMF5, 240903B8, CD84 antigen, SLAMF5

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98076-1-RR targets CD84 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1313

Product name: Recombinant Mouse CD84/SLAMF5 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-221 aa of

Sequence: KDADPMVMNGILGESVTFLLNIQEPKKIDNIAWTSQSSVAFIKPGVNKAEVTITQGTYKGRIEIIDQKYDLVIRDLRMEDAGTYKADINEENEETITKIYYLHIYRRLKTPKITQSLISSLNNTCNITLTCSVEKEEKDVTYSWSPFGEKSNVLQIVHSPMDQKLTYTCTAQNPVSNSSDSVTVQQPCTDTPSFHPRHAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD84 antigen
别名 CD84/SLAMF5, 240903B8, CD84 antigen, SLAMF5
计算分子量37 kDa
基因名称 Cd84
Gene ID (NCBI) 12523
RRIDAB_3672223
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ18PI6-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).

实验方案

Product Specific Protocols
FC protocol for CD84 antibody 98076-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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