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验证数据展示

  • WB analysis using 27873-1-AP (same clone as 27873-1-PBS)

    WB analysis using 27873-1-AP (same clone as 27873-1-PBS)

    Untreated and PNGF treated Raji cells were subjected to SDS PAGE followed by western blot with 27873-1-AP (CD83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27873-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 27873-1-AP (same clone as 27873-1-PBS)

    IHC staining of human tonsillitis using 27873-1-AP (same clone as 27873-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27873-1-AP (CD83 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27873-1-PBS in a different storage buffer formulation.

CD83 Polyclonal antibody, PBS Only

CD83 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27873-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27873-1-PBS targets CD83 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27467

Product name: Recombinant human CD83 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-144 aa of BC030830

Sequence: EVKVACSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIRNTTSCNSGTYRCTLQDPDGQRNLSGKVILRVTGCPAQRKEETFKKYRAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD83 molecule
别名 B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule
计算分子量 205 aa, 23 kDa
观测分子量 37-50kDa, 23kDa
GenBank蛋白编号BC030830
基因名称 CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
RRIDAB_3742262
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01151
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928). PNGase F (peptide N-glycosidase F) digestion reduced the 37 and 50 kDa CD83 forms to 28 kDa (PMID: 15320871).

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