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验证数据展示

  • WB analysis using 27873-1-AP

    WB analysis using 27873-1-AP

    Untreated and PNGF treated Raji cells were subjected to SDS PAGE followed by western blot with 27873-1-AP (CD83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27873-1-AP

    IHC staining of human tonsillitis using 27873-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27873-1-AP (CD83 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD83 Polyclonal antibody

CD83 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27873-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, Daudi cells, PNGF treated Daudi cells, PNGF treated Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27873-1-AP targets CD83 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27467

Product name: Recombinant human CD83 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-144 aa of BC030830

Sequence: EVKVACSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIRNTTSCNSGTYRCTLQDPDGQRNLSGKVILRVTGCPAQRKEETFKKYRAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD83 molecule
别名 B cell activation protein, B-cell activation protein, BL11, CD83 antigen, CD83 molecule
计算分子量 205 aa, 23 kDa
观测分子量 37-50kDa, 23kDa
GenBank蛋白编号BC030830
基因名称 CD83
Gene ID (NCBI) 9308
ENSEMBL Gene IDENSG00000112149
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928). PNGase F (peptide N-glycosidase F) digestion reduced the 37 and 50 kDa CD83 forms to 28 kDa (PMID: 15320871).

实验方案

Product Specific Protocols
IHC protocol for CD83 antibody 27873-1-APDownload protocol
WB protocol for CD83 antibody 27873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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