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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL488-98202

    FC experiment of human PBMCs using CL488-98202

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 Rabbit RecAb (CL488-98202, Clone: 241722G4) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL488-98202

    FC experiment of human PBMCs using CL488-98202

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 Rabbit RecAb (CL488-98202, Clone: 241722G4) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite® Plus 488 Anti-Human CD81 Rabbit Recombinant Antibody

CD81 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL488-98202

产品说明书

CloneNo. 241722G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

241722G4, 26 kDa cell surface protein TAPA-1, CD81 antigen, CD81 molecule, S5.7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 98202-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-98202 targets CD81 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 CD81 fusion protein Eg1036 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD81 molecule
别名 241722G4, 26 kDa cell surface protein TAPA-1, CD81 antigen, CD81 molecule, S5.7
计算分子量26 kDa
GenBank蛋白编号NM_004356.4
基因名称 CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP60033
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD81 antibody CL488-98202Download protocol
Standard Protocols
Click here to view our Standard Protocols