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验证数据展示

  • FC experiment of mouse splenocytes using 98129-1-RR

    FC experiment of mouse splenocytes using 98129-1-RR

    1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse CD79a Rabbit Recombinant Antibody (98129-1-RR, Clone: 241231F7) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2) (CL647-65139, Clone: RA3-6B2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of mouse splenocytes using 98129-1-RR

    FC experiment of mouse splenocytes using 98129-1-RR

    1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse CD79a Rabbit Recombinant Antibody (98129-1-RR, Clone: 241231F7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98129-1-RR

    Affinity and Kinetic Characterization of 98129-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98129-1-RR against Mouse CD79a were performed. The affinity constant is below 1 pM.

Anti-Mouse CD79a Rabbit Recombinant Antibody

CD79a Recombinant Antibody for FC (Intra)
Cat No. 98129-1-RR

产品说明书

CloneNo. 241231F7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

241231F7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98129-1-RR targets CD79a in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD79A antigen (immunoglobulin-associated alpha)
别名 241231F7
计算分子量25 kDa
GenBank蛋白编号NM_007655.3
基因名称 Cd79a
Gene ID (NCBI) 12518
RRIDAB_3672275
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP11911
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
FC protocol for CD79a antibody 98129-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols