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验证数据展示

  • FC experiment of C57BL/c mouse splenocytes using CL647-65139

    FC experiment of C57BL/c mouse splenocytes using CL647-65139

    1x10^6 C57BL/c mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45R (B220) (CL647-65139, Clone:RA3-6B2) or 0.25 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3). Cells were co-stained with PE Anti-Mouse CD3 (17A2) (PE-65077, Clone: 17A2). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL647-65139

    IF Staining of mouse splenocytes using CL647-65139

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated CD45R (B220) antibody (CL647-65139, Clone: RA3-6B2, red) at dilution of 1:400, and CoraLite®488-conjugated CD3 antibody (CL488-65077, Clone: 17A2, green).

CoraLite® Plus 647 Anti-Mouse CD45R (B220) (RA3-6B2)

CD45R (B220) Monoclonal Antibody for IF/ICC, FC
Cat No. CL647-65139

产品说明书

CloneNo. RA3-6B2

宿主/亚型

Rat / IgG2a, kappa

种属反应性

mouse

应用

IF/ICC, FC

别名

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65139-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65139 targets CD45R (B220) in IF/ICC, FC applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 mouse
免疫原 Abelson murine leukemia virus-induced pre-B tumor cells 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 B220, CD45R, Cd45, Ptprc, EC:3.1.3.48
GenBank蛋白编号BC028512
基因名称 CD45
Gene ID (NCBI) 19264
RRIDAB_2883705
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP06800
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD45R (B220) antibody CL647-65139Download protocol
FC protocol for CL Plus 647 CD45R (B220) antibody CL647-65139Download protocol
Standard Protocols
Click here to view our Standard Protocols