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验证数据展示

  • WB analysis using 31887-1-AP (same clone as 31887-1-PBS)

    WB analysis using 31887-1-AP (same clone as 31887-1-PBS)

    Mouse spleen tissue was subjected to SDS PAGE, followed by western blot with 31887-1-AP (CD79a antibody) at a dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 31887-1-AP (same clone as 31887-1-PBS)

    IHC staining of mouse spleen using 31887-1-AP (same clone as 31887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 31887-1-AP (same clone as 31887-1-PBS)

    IHC staining of mouse spleen using 31887-1-AP (same clone as 31887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

  • IF Staining of rat spleen using 31887-1-AP (same clone as 31887-1-PBS)

    IF Staining of rat spleen using 31887-1-AP (same clone as 31887-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD79a antibody (31887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31887-1-PBS in a different storage buffer formulation.

Mouse CD79a Polyclonal antibody, PBS Only

CD79a Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31887-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

B-cell antigen receptor complex-associated protein alpha chain, Mb-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31887-1-PBS targets CD79a in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg1388

Product name: Recombinant Mouse CD79A protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 29-137 aa of NM_007655.3

Sequence: LRVEGGPPSLTVNLGEEARLTCENNGRNPNITWWFSLQSNITWPPVPLGPGQGTTGQLFFPEVNKNHRGLYWCQVIENNILKRSCGTYLRVRNPVPRPFLDMGEGTKNR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD79A antigen (immunoglobulin-associated alpha)
别名 B-cell antigen receptor complex-associated protein alpha chain, Mb-1
计算分子量25 kDa
观测分子量30 kDa
GenBank蛋白编号NM_007655.3
基因名称 Cd79a
Gene ID (NCBI) 12518
RRIDAB_3670132
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP11911
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

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