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验证数据展示

  • WB analysis using 31887-1-AP

    WB analysis using 31887-1-AP

    Mouse spleen tissue was subjected to SDS PAGE followed by western blot with 31887-1-AP (CD79A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 31887-1-AP

    IHC staining of mouse spleen using 31887-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31887-1-AP

    IHC staining of mouse spleen using 31887-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat spleen using 31887-1-AP

    IF Staining of rat spleen using 31887-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD79a antibody (31887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD79a Polyclonal antibody

CD79a Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31887-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

B-cell antigen receptor complex-associated protein alpha chain, Mb-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31887-1-AP targets CD79a in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 CD79a fusion protein Eg1388 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD79A antigen (immunoglobulin-associated alpha)
别名 B-cell antigen receptor complex-associated protein alpha chain, Mb-1
计算分子量25 kDa
观测分子量30 kDa
GenBank蛋白编号NM_007655.3
基因名称 Cd79a
Gene ID (NCBI) 12518
RRIDAB_3670132
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP11911
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
WB protocol for CD79a antibody 31887-1-APDownload protocol
IHC protocol for CD79a antibody 31887-1-APDownload protocol
IF protocol for CD79a antibody 31887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols