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验证数据展示

  • FC experiment of human PBMCs using 98478-2-RR

    FC experiment of human PBMCs using 98478-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD74 Rabbit RecAb (98478-2-RR, Clone: 250520C1) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98478-2-RR

    FC experiment of human PBMCs using 98478-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD74 Rabbit RecAb (98478-2-RR, Clone: 250520C1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • IHC staining of human tonsillitis using 98478-2-RR

    IHC staining of human tonsillitis using 98478-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98478-2-RR (CD74 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 98478-2-RR

    IHC staining of human tonsillitis using 98478-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98478-2-RR (CD74 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Raji using 98478-2-RR

    IF Staining of Raji using 98478-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD74 antibody (98478-2-RR, Clone: 250520C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Anti-Human CD74 Rabbit Recombinant Antibody

CD74 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC
Cat No. 98478-2-RR

产品说明书

CloneNo. 250520C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC

别名

Class-II-associated invariant chain peptide, DHLAG, HLADG, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells
Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98478-2-RR targets CD74 in IHC, IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1945

Product name: Recombinant Human CD74 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 73-232 aa of NM_001025159.3

Sequence: QQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNADPLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQKPTDAPPKESLELEDPSSGLGVTKQDLGPVPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD74 molecule, major histocompatibility complex, class II invariant chain
别名 Class-II-associated invariant chain peptide, DHLAG, HLADG, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain
计算分子量26 kDa
GenBank蛋白编号NM_001025159.3
基因名称 CD74
Gene ID (NCBI) 972
RRIDAB_3746108
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04233-2
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165; PMID: 36712241).

实验方案

Product Specific Protocols
FC protocol for CD74 antibody 98478-2-RRDownload protocol
IF protocol for CD74 antibody 98478-2-RRDownload protocol
IHC protocol for CD74 antibody 98478-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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