• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD74 Monoclonal antibody

CD74 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D8A1

Cat No : 66390-1-Ig

Print datasheet

Synonyms

CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33

验证数据展示

  • WB analysis using 66390-1-Ig

    WB analysis using 66390-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 66390-1-Ig

    IF Staining of Raji using 66390-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD74 antibody (66390-1-Ig, Clone: 2D8A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inRaji cells, Ramos cells, Daudi cells
Positive IF detected inRaji cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66390-1-Ig targets CD74 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD74 fusion protein Ag25503 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD74 molecule, major histocompatibility complex, class II invariant chain
Synonyms CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC018726
Gene Symbol CD74
Gene ID (NCBI) 972
RRIDAB_2881766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04233
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CD74 antibody 66390-1-IgDownload protocol
IF protocol for CD74 antibody 66390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Int Immunol

Helicobacter urease suppresses cytotoxic CD8 + T cell responses through activating Myh9-dependent induction of PD-L1.

Authors - Jian Wu
View Article
IF

Neural Regen Res

Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect

Authors - Piniel Alphayo Kambey
View Article
humanIF

Nat Commun

Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment

Authors - Xiaojian Lu
View Article
humanWB

Am J Physiol Cell Physiol

WISP1 induces the expression of macrophage migration inhibitory factor (MIF) in human lung fibroblasts through Src kinases and EGFR-activated signaling pathways

Authors - Maria-Elpida Christopoulou
View Article