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Species Reactivity
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Species Reactivity
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验证数据展示

  • FC experiment of human PBMCs using 98566-1-RR

    FC experiment of human PBMCs using 98566-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 Rabbit RecAb (98566-1-RR, Clone:251250H3) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed.

  • FC experiment of human PBMCs using 98566-1-RR

    FC experiment of human PBMCs using 98566-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 Rabbit RecAb (98566-1-RR, Clone: 251250H3) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

Anti-Human CD73 Rabbit Recombinant Antibody

CD73 Uni-rAb® Recombinant Antibody for FC
Cat No. 98566-1-RR

产品说明书

CloneNo. 251250H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98566-1-RR targets CD73 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg2974

Product name: recombinant human NT5E protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-547 aa of NM_002526.3

Sequence: WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase
计算分子量63 kDa
GenBank蛋白编号NM_002526.3
基因名称 CD73
Gene ID (NCBI) 4907
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21589-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

实验方案

Product Specific Protocols
FC protocol for CD73 antibody 98566-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols