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验证数据展示

  • FC experiment of mouse splenocytes using 98199-1-RR

    FC experiment of mouse splenocytes using 98199-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

  • FC experiment of mouse splenocytes using 98199-1-RR

    FC experiment of mouse splenocytes using 98199-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98199-1-RR

    Affinity and Kinetic Characterization of 98199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98199-1-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

Anti-Mouse CD73 Rabbit Recombinant Antibody

CD73 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98199-1-RR

产品说明书

CloneNo. 241541G9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98199-1-RR targets CD73 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5' nucleotidase, ecto
别名 Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase
计算分子量64kDa
GenBank蛋白编号NM_011851.4
基因名称 Nt5e
Gene ID (NCBI) 23959
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ61503
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for CD73 antibody 98199-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols