验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
PE-FcA98204 targets CD7 in FC applications and shows reactivity with human samples.
经测试应用 | FC Application Description |
经测试反应性 | human |
免疫原 | CD7 fusion protein Eg3034 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | CD7 molecule |
别名 | CD7 molecule, GP40, LEU-9, T cell antigen CD7, T cell leukemia antigen |
计算分子量 | 25 kDa |
GenBank蛋白编号 | NM_006137.7 |
基因名称 | CD7 |
Gene ID (NCBI) | 924 |
ENSEMBL Gene ID | ENSG00000173762 |
偶联类型 | PE Fluorescent Dye |
最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).
实验方案
Product Specific Protocols | |
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FC protocol for PE CD7 antibody PE-FcA98204 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |