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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)

    WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

  • WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)

    WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD7 antibody included

CD7 Monoclonal antibody, PBS Only

CD7 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60209-2-PBS

产品说明书

CloneNo. 2A4E6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

2A4E6, CD7 molecule, GP40, LEU-9, T cell antigen CD7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.

产品信息

60209-2-PBS targets CD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CD7 fusion protein Ag1805 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD7 molecule
别名 2A4E6, CD7 molecule, GP40, LEU-9, T cell antigen CD7
计算分子量 240 aa, 25 kDa
观测分子量 34-36 kDa
GenBank蛋白编号BC009293
基因名称 CD7
Gene ID (NCBI) 924
ENSEMBL Gene IDENSG00000173762
RRIDAB_2881355
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP09564
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).