验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11320-1-AP targets CD7 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, IF-P, ELISA Application Description |
经测试反应性 | human |
免疫原 | CD7 fusion protein Ag0486 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | CD7 molecule |
别名 | CD7 molecule, GP40, LEU-9, T cell antigen CD7, T cell leukemia antigen |
计算分子量 | 240 aa, 25 kDa |
GenBank蛋白编号 | BC009293 |
基因名称 | CD7 |
Gene ID (NCBI) | 924 |
ENSEMBL Gene ID | ENSG00000173762 |
RRID | AB_2877758 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P09564 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).
实验方案
Product Specific Protocols | |
---|---|
IHC protocol for CD7 antibody 11320-1-AP | Download protocol |
IF protocol for CD7 antibody 11320-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |