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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 98173-2-RR

    FC experiment of human PBMCs using 98173-2-RR

    1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone: 241552F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD4 (RPA-T4). Cells were not fixed.

  • FC experiment of human PBMCs using 98173-2-RR

    FC experiment of human PBMCs using 98173-2-RR

    1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone: 241552F1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone:241552F1)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of Cnyo PBMCs using 98173-2-RR

    FC experiment of Cnyo PBMCs using 98173-2-RR

    1x10^6 Cnyo PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit RecAb (98173-2-RR, Clone:241552G10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • FC experiment of Cnyo PBMCs using 98173-2-RR

    FC experiment of Cnyo PBMCs using 98173-2-RR

    1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit RecAb (98173-2-RR, Clone: 241552G10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

Anti-Human CD69 Rabbit Recombinant Antibody

CD69 Uni-rAb® Recombinant Antibody for FC
Cat No. 98173-2-RR

产品说明书

CloneNo. 241552G10

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey, non-human primates

应用

FC

别名

Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inPHA treated human PBMCs, cynomolgus PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98173-2-RR targets CD69 in FC applications and shows reactivity with human, monkey, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, monkey, non-human primates
免疫原

CatNo: Eg1791

Product name: Recombinant Human CD69 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 62-199 aa of NM_001781.2

Sequence: SVGQYNCPGQYTFSMPSDSHVSSCSEDWVGYQRKCYFISTVKRSWTSAQNACSEHGATLAVIDSEKDMNFLKRYAGREEHWVGLKKEPGHPWKWSNGKEFNNWFNVTGSDKCVFLKNTEVSSMECEKNLYWICNKPYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD69 molecule
别名 Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule
计算分子量23 kDa
GenBank蛋白编号NM_001781.2
基因名称 CD69
Gene ID (NCBI) 969
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ07108
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

实验方案

Product Specific Protocols
FC protocol for CD69 antibody 98173-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols