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验证数据展示

  • WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

    WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD68 antibody included
  • IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IF Staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83014-5-RR

    Affinity and Kinetic Characterization of 83014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

Mouse CD68 Recombinant monoclonal antibody, PBS Only

CD68 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 83014-5-PBS

产品说明书

CloneNo. 230504G10

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

Scard1, gp110, CD68 antigen, 230504G10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

产品信息

83014-5-PBS targets CD68 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg0787

Product name: Recombinant mouse CD68 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-291 aa of NM_001291058.1

Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD68 antigen
别名 Scard1, gp110, CD68 antigen, 230504G10
计算分子量35 kDa
观测分子量100-120 kDa
GenBank蛋白编号NM_001291058.1
基因名称 Cd68
Gene ID (NCBI) 12514
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP31996-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

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