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验证数据展示

  • WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

    WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD68 antibody included
  • IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • IF Staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

    IF Staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83014-5-RR

    Affinity and Kinetic Characterization of 83014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

Mouse CD68 Recombinant monoclonal antibody, PBS Only

CD68 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 83014-5-PBS

产品说明书

CloneNo. 230504G10

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

230504G10, CD68 antigen, gp110, Scard1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

产品信息

83014-5-PBS targets CD68 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg0787

Product name: Recombinant mouse CD68 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-291 aa of NM_001291058.1

Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD68 antigen
别名 230504G10, CD68 antigen, gp110, Scard1
计算分子量35 kDa
观测分子量100-120 kDa
GenBank蛋白编号NM_001291058.1
基因名称 Cd68
Gene ID (NCBI) 12514
RRIDAB_3670757
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP31996-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. Due to heavy glycosylation, the apparent molecular mass of CD68 detected by Western blot is generally observed between 80 and 140 kDa, far exceeding its unmodified core protein weight of ~34-37 kDa.

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