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验证数据展示

  • FC experiment of human PBMCs using 65593-1-MR

    FC experiment of human PBMCs using 65593-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone: KP1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.

  • FC experiment of human PBMCs using 65593-1-MR

    FC experiment of human PBMCs using 65593-1-MR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD68 Mouse IgG2a Recombinant Antibody (65593-1-MR, Clone:KP1) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. Monocytes were gated.

Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody

CD68 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 65593-1-MR

产品说明书

CloneNo. KP1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC (Intra)

别名

CD68 molecule, GP110, KP1, Macrosialin, SCARD1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65593-1-MR targets CD68 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原

N/A

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD68 molecule
别名 CD68 molecule, GP110, KP1, Macrosialin, SCARD1
计算分子量 37 kDa
GenBank蛋白编号BC015557
基因名称 CD68
Gene ID (NCBI) 968
RRIDAB_3670342
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP34810
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for CD68 antibody 65593-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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