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验证数据展示

  • WB analysis using 30929-1-AP

    WB analysis using 30929-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30929-1-AP (CD68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 30929-1-AP

    IHC staining of mouse spleen using 30929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IHC staining of mouse spleen using 30929-1-AP

    IHC staining of mouse spleen using 30929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30929-1-AP (CD68 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD68 antibody included
  • IF Staining of mouse spleen using 30929-1-AP

    IF Staining of mouse spleen using 30929-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 30929-1-AP

    IF Staining of mouse spleen using 30929-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (30929-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD68 Polyclonal antibody

CD68 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 30929-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CD68 antigen, gp110, Scard1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNR8383 cells, RAW 264.7 cells
Positive IHC detected inmouse spleen tissue, mouse liver tissue, rat liver tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

30929-1-AP targets CD68 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IHC
经测试反应性 mouse, rat
文献引用反应性mouse, rat
免疫原

CatNo: Eg0787

Product name: Recombinant mouse CD68 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-291 aa of NM_001291058.1

Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD68 antigen
别名 CD68 antigen, gp110, Scard1
计算分子量35 kDa
观测分子量90-100 kDa
GenBank蛋白编号NM_001291058.1
基因名称 Cd68
Gene ID (NCBI) 12514
RRIDAB_3086426
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31996-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
IF protocol for CD68 antibody 30929-1-APDownload protocol
IHC protocol for CD68 antibody 30929-1-APDownload protocol
WB protocol for CD68 antibody 30929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Nanobiotechnology

Biomimetic mesenchymal stem cell membrane-coated nanoparticle delivery of MKP5 inhibits hepatic fibrosis through the IRE/XBP1 pathway

Authors - Yafei Tian
View Article
ratIHC

J Orthop Res

Optimal Timing for Initiating Postoperative Mobilization for Healing Enthesis in Onto-Surface Repair

Authors - Takuma Naka
View Article
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