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验证数据展示

  • FC experiment of HL-60 using CL488-25682

    FC experiment of HL-60 using CL488-25682

    1x10^6 HL-60 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CD63 Polyclonal antibody (CL488-25682)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CD63 Polyclonal antibody

CD63 Polyclonal Antibody for
Cat No. CL488-25682

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

别名

Ocular melanoma-associated antigen, Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHL-60 cells
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 25682-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25682 targets CD63 in applications and shows reactivity with human samples.

经测试应用 Application Description
经测试反应性 human
免疫原 CD63 fusion protein Ag19690 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD63 molecule
别名 Ocular melanoma-associated antigen, Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen
计算分子量 26 kDa
GenBank蛋白编号BC002349
基因名称 CD63
Gene ID (NCBI) 967
RRIDAB_2919203
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08962
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD63 antibody CL488-25682Download protocol
Standard Protocols
Click here to view our Standard Protocols