CD63 Polyclonal antibody
CD63 Polyclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (5)
Applications
IF-P, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
311
验证数据展示
经过测试的应用
Positive WB detected in | human urine exosomes tissue, human serum exosomes |
Positive IHC detected in | human malignant melanoma tissue, human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human malignant melanoma tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25682-1-AP targets CD63 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF-P, IHC, WB, ELISA |
Cited Applications | WB, IP, IF, FC, IHC |
Tested Reactivity | human |
Cited Reactivity | human, monkey, rabbit, zebrafish, pig, canine |
Immunogen | CD63 fusion protein Ag19690 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD63 molecule |
Synonyms | CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 28-35 kDa |
GenBank Accession Number | BC002349 |
Gene Symbol | CD63 |
Gene ID (NCBI) | 967 |
RRID | AB_2783831 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08962 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CD63 antibody 25682-1-AP | Download protocol |
IHC protocol for CD63 antibody 25682-1-AP | Download protocol |
IF protocol for CD63 antibody 25682-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Adv Sci (Weinh) Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury | ||
J Extracell Vesicles Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. | ||
Small Methods Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers. | ||
J Exp Clin Cancer Res Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization |