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验证数据展示

  • WB analysis of human plasma using 86638-1-RR (same clone as 86638-1-PBS)

    WB analysis of human plasma using 86638-1-RR (same clone as 86638-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 86638-1-RR (CD5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86638-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected CHO-K1 using 86638-1-RR (same clone as 86638-1-PBS)

    IF Staining of Transfected CHO-K1 using 86638-1-RR (same clone as 86638-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed transfected CHO-K1 using CD5L antibody (86638-1-RR, Clone: 250651G1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 86638-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86638-1-RR

    Affinity and Kinetic Characterization of 86638-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86638-1-RR against Human CD5L were performed. The affinity constant is 0.487 nM.

CD5L Recombinant monoclonal antibody, PBS Only

CD5L Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86638-1-PBS

产品说明书

CloneNo. 250651G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

AIM, API6, Apoptosis inhibitor expressed by macrophages, CD5 antigen-like, CT-2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86638-1-PBS targets CD5L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3129

Product name: Recombinant Human CD5L protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-347 aa of NM_005894.2

Sequence: SPSGVRLVGGLHRCEGRVEVEQKGQWGTVCDDGWDIKDVAVLCRELGCGAASGTPSGILYEPPAEKEQKVLIQSVSCTGTEDTLAQCEQEEVYDCSHDEDAGASCENPESSFSPVPEGVRLADGPGHCKGRVEVKHQNQWYTVCQTGWSLRAAKVVCRQLGCGRAVLTQKRCNKHAYGRKPIWLSQMSCSGREATLQDCPSGPWGKNTCNHDEDTWVECEDPFDLRLVGGDNLCSGRLEVLHKGVWGSVCDDNWGEKEDQVVCKQLGCGKSLSPSFRDRKCYGPGVGRIWLDNVRCSGEEQSLEQCQHRFWGFHDCTHQEDVAVICSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD5 molecule-like
别名 AIM, API6, Apoptosis inhibitor expressed by macrophages, CD5 antigen-like, CT-2
计算分子量38 kDa
观测分子量50 kDa
GenBank蛋白编号NM_005894.2
基因名称 CD5L
Gene ID (NCBI) 922
RRIDAB_3745022
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43866
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).

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