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验证数据展示

  • FC experiment of human PBMCs using CL647-98099

    FC experiment of human PBMCs using CL647-98099

    1x10^6 human PBMCs were surface stained with PE Anti-Human CD3, and 5 ul CoraLite® Plus 647 Anti-Human CD59 Rabbit RecAb (CL647-98099, Clone: 241311A4) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL647-98099

    FC experiment of human PBMCs using CL647-98099

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD59 Rabbit RecAb (CL647-98099, Clone: 241311A4) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

CoraLite® Plus 647 Anti-Human CD59 Rabbit Recombinant Antibody

CD59 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL647-98099

产品说明书

CloneNo. 241311A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

20 kDa homologous restriction factor, 241311A4, CD 59, CD59 antigen, CD59 glycoprotein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98099-1-PBS

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98099 targets CD59 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD59 molecule, complement regulatory protein
别名 20 kDa homologous restriction factor, 241311A4, CD 59, CD59 antigen, CD59 glycoprotein
计算分子量 19 kDa
GenBank蛋白编号BC001506
基因名称 CD59
Gene ID (NCBI) 966
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13987
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD59 antibody CL647-98099Download protocol
Standard Protocols
Click here to view our Standard Protocols