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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using CL555-65264

    FC experiment of human PBMCs using CL555-65264

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 5 ul CoraLite® Plus 555 Anti-Human CD56 (CL555-65264, Clone: B-A19) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CoraLite® Plus 555 Anti-Human NCAM1/CD56 (B-A19)

NCAM1/CD56 Monoclonal Antibody for FC
Cat No. CL555-65264

产品说明书

CloneNo. B-A19

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC

别名

CD56, NCAM1, NCAM 1, NCAM, N CAM 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 555
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy NCAM1/CD56 Ready-To-Use IHC Kit. NCAM1/CD56 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65264-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65264 targets NCAM1/CD56 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neural cell adhesion molecule 1
别名 CD56, NCAM1, NCAM 1, NCAM, N CAM 1
GenBank蛋白编号BC014205
基因名称 NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
RRIDAB_3084617
偶联类型 CoraLite® Plus 555 Fluorescent Dye
最大激发/发射波长554 nm / 570 nm
形式Liquid
纯化方式Affinity purification
UNIPROT IDP13591
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 NCAM1/CD56 antibody CL555-65264Download protocol
Standard Protocols
Click here to view our Standard Protocols