验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
FITC-98228 targets CD55 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2124 Product name: Recombinant Mouse CD55 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 35-361 aa of NM_010016.3 Sequence: DCGPPPDIPNARPILGRHSKFAEQSKVAYSCNNGFKQVPDKSNIVVCLENGQWSSHETFCEKSCVAPERLSFASLKKEYLNMNFFPVGTIVEYECRPGFRKQPPLPGKATCLEDLVWSPVAQFCKKKSCPNPKDLDNGHINIPTGILFGSEINFSCNPGYRLVGVSSTFCSVTGNTVDWDDEFPVCTEIHCPEPPKINNGIMRGESDSYTYSQVVTYSCDKGFILVGNASIYCTVSKSDVGQWSSPPPRCIEKSKVPTKKPTINVPSTGTPSTPQKPTTESVPNPGDQPTPQKPSTVKVSATQHVPVTKTTVRHPIRTSTDKGEPNT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD55 antigen |
| 别名 | 241808D11, Cd55a, Complement decay-accelerating factor, GPI-anchored, Daf, Daf1 |
| 计算分子量 | 43kDa |
| GenBank蛋白编号 | NM_010016.3 |
| 基因名称 | CD55 |
| Gene ID (NCBI) | 13136 |
| 偶联类型 | FITC Plus Fluorescent Dye |
| 最大激发/发射波长 | 495 nm / 524 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q61475 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD55 antibody FITC-98228 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
