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FITC Anti-Mouse CD54 (YN1/1.7.4)

CD54 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

YN1/1.7.4

Cat No : FITC-65066

Print datasheet

Synonyms

CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2

验证数据展示

  • FC experiment of mouse splenocytes using FITC-65066

    FC experiment of mouse splenocytes using FITC-65066

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 1.00 ug FITC Anti-Mouse CD54 (FITC-65066, Clone: YN1/1.7.4) (red) or 1.00 ug isotype control antibody (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65066 targets CD54 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen Mouse NS-1 cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2
GenBank Accession NumberBC008626
Gene Symbol Icam1
Gene ID (NCBI) 15894
RRIDAB_2883756
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths494 nm / 520 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP13597
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for FITC CD54 antibody FITC-65066Download protocol
Standard Protocols
Click here to view our Standard Protocols