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验证数据展示

  • WB analysis of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

    WB analysis of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 83543-7-RR (CD5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • IHC staining of human tonsil using 83543-7-RR (same clone as 83543-7-PBS)

    IHC staining of human tonsil using 83543-7-RR (same clone as 83543-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • IHC staining of human tonsil using 83543-7-RR (same clone as 83543-7-PBS)

    IHC staining of human tonsil using 83543-7-RR (same clone as 83543-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 83543-7-RR (CD5 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

    IF Staining of Jurkat using 83543-7-RR (same clone as 83543-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD5 antibody (83543-7-RR, Clone: 240428G2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83543-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83543-7-RR

    Affinity and Kinetic Characterization of 83543-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83543-7-RR against Human CD5 were performed. The affinity constant is 2.09 nM.

CD5 Recombinant monoclonal antibody, PBS Only

CD5 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83543-7-PBS

产品说明书

CloneNo. 240428G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

240428G2, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83543-7-PBS targets CD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1119

Product name: Recombinant Human CD5 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 25-372 aa of NM_014207.4

Sequence: RLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVTYTPQSSIICYGQLGSFSNCSHSRNDMCHSLGLTCLEPQKTTPPTTRPPPTTTPEPTAPPRLQLVAQSGGQHCAGVVEFYSGSLGGTISYEAQDKTQDLENFLCNNLQCGSFLKHLPETEAGRAQDPGEPREHQPLPIQWKIQNSSCTSLEHCFRKIKPQKSGRVLALLCSGFQPKVQSRLVGGSSICEGTVEVRQGAQWAALCDSSSARSSLRWEEVCREQQCGSVNSYRVLDAGDPTSRGLFCPHQKLSQCHELWERNSYCKKVFVTCQDPNP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD5 molecule
别名 240428G2, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1
计算分子量55 kDa
观测分子量60 kDa
GenBank蛋白编号NM_014207.4
基因名称 CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP06127
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

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