验证数据展示
经过测试的应用
Positive FC detected in | human peripheral blood leukocytes |
推荐稀释比
应用 | 推荐稀释比 |
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Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
PE-FcA98170 targets CD48 in FC applications and shows reactivity with human samples.
经测试应用 | FC Application Description |
经测试反应性 | human |
免疫原 | CD48 fusion protein Eg1005 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | CD48 molecule |
别名 | BLAST, BLAST1, B-lymphocyte activation marker BLAST-1, CD48 antigen, CD48 molecule |
计算分子量 | 43 kDa |
GenBank蛋白编号 | BC016182 |
基因名称 | CD48 |
Gene ID (NCBI) | 962 |
偶联类型 | PE Fluorescent Dye |
最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.
实验方案
Product Specific Protocols | |
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FC protocol for PE CD48 antibody PE-FcA98170 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |