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CoraLite®594 Anti-Rat CD45RA (OX-33)

CD45RA Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

OX-33

Cat No : CL594-65234

Print datasheet

Synonyms

CD45, CD45RA, L CA, Lca, Ptprc, RT7, T200

验证数据展示

  • FC experiment of rat splenocytes using CL594-65234

    FC experiment of rat splenocytes using CL594-65234

    1X10^6 rat splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Rat CD45RA (CL594-65234, Clone: OX-33), or 0.5 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21). Cells were not fixed.

  • FC experiment of rat splenocytes using CL594-65234

    FC experiment of rat splenocytes using CL594-65234

    1X10^6 rat splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Rat CD45RA (CL594-65234, Clone:OX-33) (red), or 0.5 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inrat splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65234-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65234 targets CD45RA in FC applications and shows reactivity with Rat samples.

Tested Applications FC
Tested Reactivity Rat
Immunogen Leukocyte common antigen purified from rat splenocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms CD45, CD45RA, L CA, Lca, Ptprc, RT7, T200
Calculated Molecular Weight143 kDa
GenBank Accession NumberXM_006249910
Gene Symbol Ptprc
Gene ID (NCBI) 24699
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodN/A
UNIPROT IDP04157
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
FC protocol for CL594 CD45RA antibody CL594-65234Download protocol
Standard Protocols
Click here to view our Standard Protocols