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验证数据展示

  • FC experiment of human PBMCs using 65226-1-Ig

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3, FITC Anti-human CD45RO, and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • FC experiment of human PBMCs using 65226-1-Ig

    FC experiment of human PBMCs using 65226-1-Ig

    1X10^6 human PBMCs were surface co-stained with PE Anti-human CD3 and 0.2 ug Anti-Human CD45RA (65226-1-Ig, Clone: F8-11-13) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

  • IHC staining of human tonsillitis using 65226-1-Ig

    IHC staining of human tonsillitis using 65226-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 65226-1-Ig (CD45RA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD45 antibody included
  • IF Staining of human tonsillitis using 65226-1-Ig

    IF Staining of human tonsillitis using 65226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD45RA antibody (65226-1-Ig, Clone: F8-11-13 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Anti-Human CD45RA (F8-11-13)

CD45RA Monoclonal Antibody for IHC, IF-P, FC
Cat No. 65226-1-Ig

产品说明书

CloneNo. F8-11-13

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

IHC, IF-P, FC

别名

CD45, EC:3.1.3.48, F8-11-13, GP180, L CA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65226-1-Ig targets CD45RA in IHC, IF-P, FC applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, FC Application Description
经测试反应性 human
免疫原 Human T lymphocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 CD45, EC:3.1.3.48, F8-11-13, GP180, L CA
计算分子量 1304 aa, 147 kDa
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_3084907
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08575
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
IHC protocol for CD45RA antibody 65226-1-IgDownload protocol
IF protocol for CD45RA antibody 65226-1-IgDownload protocol
FC protocol for CD45RA antibody 65226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols