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验证数据展示

  • FC experiment of mouse splenocytes using CL700-98035

    FC experiment of mouse splenocytes using CL700-98035

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®700 Anti-Mouse CD45 Rabbit RecAb (CL700-98035, Clone: 240356D1) or CoraLite®700 Rabbit IgG Isotype Control Recombinant Antibody (CL700-98136, Clone: 240953C9). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL700-98035

    FC experiment of mouse splenocytes using CL700-98035

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®700 Anti-Mouse CD45 Rabbit RecAb (CL700-98035, Clone: 240356D1) (red) or CoraLite®700 Rabbit IgG Isotype Control Recombinant Antibody (CL700-98136, Clone: 240953C9) (blue). Cells were not fixed.

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CoraLite®700 Anti-Mouse CD45 Rabbit Recombinant Antibody

CD45 Uni-rAb® Recombinant Antibody for FC
Cat No. CL700-98035

产品说明书

CloneNo. 240356D1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Ptprc, 240356D1, EC:3.1.3.48, LCA, L-CA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®700
规格价格库存

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经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98035-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL700-98035 targets CD45 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg0845

Product name: Recombinant Mouse CD45 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-566 aa of NM_001111316.2

Sequence: QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 Ptprc, 240356D1, EC:3.1.3.48, LCA, L-CA
计算分子量145 kDa
GenBank蛋白编号NM_001111316.2
基因名称 CD45
Gene ID (NCBI) 19264
偶联类型 CoraLite®700 Fluorescent Dye
最大激发/发射波长702 nm / 723 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06800-4
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
FC protocol for CL700 CD45 antibody CL700-98035Download protocol
Standard Protocols
Click here to view our Standard Protocols