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CoraLite® Plus 488 Anti-Human CD45 (2D1)

CD45 Monoclonal Antibody for FC, IF/ICC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D1

Cat No : CL488-65082

Print datasheet

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

验证数据展示

  • FC experiment of human PBMCs using CL488-65082

    FC experiment of human PBMCs using CL488-65082

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD45 (CL488-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

  • IF Staining of human peripheral blood mononuclear cells using CL488-65082

    IF Staining of human peripheral blood mononuclear cells using CL488-65082

    Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD45 (CL488-65082, Clone: 2D1) at dilution of 1:400.

经过测试的应用

Positive IF detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65082-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65082 targets CD45 in IF, FC applications and shows reactivity with Human samples.

Tested Applications FC, IF/ICC
Tested Reactivity Human
Immunogen Human PBMC 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
GenBank Accession NumberBC014239
Gene Symbol PTPRC
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2883166
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD45 antibody CL488-65082Download protocol
FC protocol for CL Plus 488 CD45 antibody CL488-65082Download protocol
Standard Protocols
Click here to view our Standard Protocols