CoraLite® Plus 647 Anti-Mouse CD45.2 Rabbit Recombinant Antibody

CD45.2 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL647-98168

产品说明书

CloneNo. 240922E12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

Cd45, Ptprc, 240922E12, EC:3.1.3.48, LCA

缓冲液配方:  PBS and Azide
PBS and Azide
规格价格库存


经过测试的应用

Positive FC detected inC57BL/6 mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98168-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98168 targets CD45.2 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 Cd45, Ptprc, 240922E12, EC:3.1.3.48, LCA
基因名称 CD45
Gene ID (NCBI) 19264
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD45.2 antibody CL647-98168Download protocol
Standard Protocols
Click here to view our Standard Protocols
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