验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84637 targets CD45.2 in IF-P applications and shows reactivity with mouse samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2467 Product name: recombinant mouse CD45.2-ly5b-Domain3 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Sequence: ATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | Cd45, Ptprc, 241543F9, EC:3.1.3.48, LCA |
| 观测分子量 | 200-220 kDa |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 19264 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD45.2 antibody CL488-84637 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
