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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84637-1-RR (same clone as 84637-1-PBS)

    WB analysis using 84637-1-RR (same clone as 84637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    IHC staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    IHC staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84637-1-RR (CD45.2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    IF Staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD45.2 antibody (84637-1-RR, Clone: 241543F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    IF Staining of mouse spleen using 84637-1-RR (same clone as 84637-1-PBS)

    Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse spleen tissue using CD45.2 antibody (84637-1-RR, Clone: 241543F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84637-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84637-1-RR

    Affinity and Kinetic Characterization of 84637-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84637-1-RR against Mouse CD45.2 were performed. The affinity constant is 23.9 nM.

Mouse CD45.2 Recombinant antibody, PBS Only

CD45.2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA
Cat No. 84637-1-PBS

产品说明书

CloneNo. 241543F9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, IF-Fro, Indirect ELISA

别名

Cd45, Ptprc, 241543F9, B220, EC:3.1.3.48

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84637-1-PBS targets CD45.2 in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, IF-Fro, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 CD45.2 fusion protein Eg2467 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 Cd45, Ptprc, 241543F9, B220, EC:3.1.3.48
观测分子量200-220 kDa
基因名称 CD45
Gene ID (NCBI) 19264
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).