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验证数据展示

  • WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD43 antibody (66224-1-Ig, Clone: 2A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

CD43 Monoclonal antibody, PBS Only

CD43 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66224-1-PBS

产品说明书

CloneNo. 2A11D6

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

2A11D6, CD43 cytoplasmic tail, Galactoglycoprotein, GALGP, GPL115

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.

产品信息

66224-1-PBS targets CD43 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23595

Product name: Recombinant human SPN protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 101-400 aa of BC012350

Sequence: KMSSVPQETPHATSHPAVPITANSLGSHTVTGGTITTNSPETSSRTSGAPVTTAASSLETSRGTSGPPLTMATVSLETSKGTSGPPVTMATDSLETSTGTTGPPVTMTTGSLEPSSGASGPQVSSVKLSTMMSPTTSTNASTVPFRNPDENSRGMLPVAVLVALLAVIVLVALLLLWRRRQKRRTGALVLSRGGKRNGVVDAWAGPAQVPEEGAVTVTVGGSGGDKGSGFPDGEGSSRRPTLTTFFGRRKSRQGSLAMEELKSGSGPSLKGEEEPLVASEDGAVDAPAPDEPEGGDGAAP

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 sialophorin
别名 2A11D6, CD43 cytoplasmic tail, Galactoglycoprotein, GALGP, GPL115
计算分子量 400 aa, 43 kDa
观测分子量 115 kDa
GenBank蛋白编号BC012350
基因名称 CD43
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
RRIDAB_2881615
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP16150
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

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