CD43 Monoclonal antibody

CD43 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A11D6

Cat No : 66224-1-Ig

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Synonyms

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN



经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells, human tonsillitis tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

产品信息

66224-1-Ig targets CD43 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IHC, WB, ELISA
Tested Reactivity human
Immunogen CD43 fusion protein Ag23595 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sialophorin
Synonyms CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN
Calculated molecular weight 400 aa, 43 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC012350
Gene symbol SPN
UniprotP16150
Gene ID (NCBI) 6693
RRIDAB_2881615
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

实验方案

Product Specific Protocols
WB protocol for CD43 antibody 66224-1-IgDownload protocol
IHC protocol for CD43 antibody 66224-1-IgDownload protocol
IF protocol for CD43 antibody 66224-1-IgDownload protocol
FC protocol for CD43 antibody 66224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols