验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-SF98191 targets CD40 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg1797 Product name: Recombinant Human CD40/TNFRSF5 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 21-193 aa of NM_001250.6 Sequence: EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQDRLR 种属同源性预测 |
| 宿主/类型 | Rabbit / scFv |
| 抗体类别 | Recombinant |
| 产品类型 | Single-chain variable fragment antibody (scFv) |
| 全称 | CD40 molecule, TNF receptor superfamily member 5 |
| 别名 | Bp50, CD40L receptor, p50, TNFRSF5 |
| 计算分子量 | 31kDa |
| GenBank蛋白编号 | NM_001250.6 |
| 基因名称 | CD40 |
| Gene ID (NCBI) | 958 |
| ENSEMBL Gene ID | ENSG00000101017 |
| RRID | AB_3748943 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| 来源 | Anti-Human CD40 scFv from clone 241760A9, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus. |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD40, also named as TNFRSF5 and Bp50, is a receptor for TNFSF5/CD40LG. It is a member of the TNFR superfamily and is responsible for the efficient activation of cell-mediated and humoral immune responses via activated T cells and B cells, respectively. Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD40 antibody CL647-SF98191 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
