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验证数据展示

  • FC experiment of mouse splenocytes using CL488-65141

    FC experiment of mouse splenocytes using CL488-65141

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD4 (RM4-5) (CL488-65141, Clone:RM4-5) or 0.25 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL488-65141

    IF Staining of mouse splenocytes using CL488-65141

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65141, Clone: RM4-5) at dilution of 1:500.

CoraLite® Plus 488 Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65141

产品说明书

CloneNo. RM4-5
引用文献(2)

宿主/亚型

Rat / IgG2a, kappa

种属反应性

Mouse and More (1)

应用

IF/ICC, FC and More (1)

别名

Cd4, CD4 antigen, L3T4, Ly 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

CL488-65141 targets CD4 in IF/ICC, FC applications and shows reactivity with Mouse samples.

经测试应用 IF/ICC, FC Application Description
文献引用应用IF
经测试反应性 Mouse
文献引用反应性mouse
免疫原 BALB/c mouse thymocytes 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD4 antigen
别名 Cd4, CD4 antigen, L3T4, Ly 4
GenBank蛋白编号BC039137
基因名称 Cd4
Gene ID (NCBI) 12504
RRIDAB_2883201
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD4 antibody CL488-65141Download protocol
FC protocol for CL Plus 488 CD4 antibody CL488-65141Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Nat Commun

In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis

Authors - Xinyan Zhou
View Article
mouseIF

Cell Commun Signal

Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma

Authors - Yuqi Yang
View Article