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验证数据展示

  • Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP50077-1

    Sample test of MP50077-1

    The mean CD4 concentration was determined to be 5.16 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

  • Sample test of MP50077-1

    Sample test of MP50077-1

    Serum of sixteen individual healthy human donors was measured. The human CD4 concentration of detected samples was determined to be 101.4 pg/mL with a range of 12.7 - 255.0 pg/mL.

CD4 Monoclonal antibody, PBS Only (Detector)

CD4 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68688-1-PBS

产品说明书

CloneNo. 1E11B4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Sandwich ELISA, Indirect ELISA, Sample test

别名

CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68688-1-PBS targets CD4 as part of a matched antibody pair:

MP50077-1: 65147-1-PBS capture and 68688-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Eg0333

Product name: Recombinant Human CD4 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-396 aa of BC025782

Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
亲和力
KD=1.39 x 10-10M
KOff=4.36 x 10-6M
KOn=3.13 x 104M
全称 CD4 molecule
别名 CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
计算分子量 55 kDa
GenBank蛋白编号BC025782
基因名称 CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP01730
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C