CoraLite® Plus 488-conjugated CD39/ENTPD1 Monoclonal antibody
CD39/ENTPD1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D2H6
验证数据展示
经过测试的应用
Positive FC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 60323-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-60323 targets CD39/ENTPD1 in FC (Intra) applications and shows reactivity with human samples.
Tested Applications | FC (Intra) |
Tested Reactivity | human |
Immunogen | CD39/ENTPD1 fusion protein Ag6165 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | ectonucleoside triphosphate diphosphohydrolase 1 |
Synonyms | ATPDase, CD39, DKFZp686D194, DKFZp686I093, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, FLJ40921, FLJ40959, NTPDase 1 |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | BC047664 |
Gene Symbol | ENTPD1 |
Gene ID (NCBI) | 953 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P49961 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD39/ENTPD1 antibody CL488-60323 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |