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Host
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

    FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65622-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

    FC experiment of human PBMCs using 65622-1-MR (same clone as 65622-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65622-1-PBS in a different storage buffer formulation.

Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody, PBS Only

CD35 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65622-1-PBS

产品说明书

CloneNo. E11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

KN, E11, CR1, Complement receptor type 1, C3BR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.

产品信息

65622-1-PBS targets CD35 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 complement component (3b/4b) receptor 1 (Knops blood group)
别名 KN, E11, CR1, Complement receptor type 1, C3BR
计算分子量 224 kDa
GenBank蛋白编号NM_000573
基因名称 CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.