验证数据展示
经过测试的应用
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Transfected CHO cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
BK00003 targets CD34 in IHC, FC applications and shows reactivity with human samples.
| 经测试应用 | IHC, FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD34 molecule |
| 别名 | CD34 molecule |
| 计算分子量 | 41 kDa |
| GenBank蛋白编号 | BC039146 |
| 基因名称 | CD34 |
| Gene ID (NCBI) | 947 |
| 偶联类型 | Unconjugated |
| 来源 | Purified from CHO cell supernatant in an animal-free facility |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 纯度 | ≥98% (HPLC), ≥98% (SDS-PAGE) |
| 无菌性 | 0.22 µm filtration |
| UNIPROT ID | P28906 |
| 储存缓冲液 | Sterile PBS, pH 7.3. |
| 储存条件 | For long-term storage, store at -80°C. Avoid repeated freeze-thaw cycles. For short-term use, store at 2-8°C for up to 2 weeks. |
| 内毒素 | <0.05 EU/mg |
背景介绍
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD34 antibody BK00003 | Download protocol |
| IHC protocol for CD34 antibody BK00003 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
