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验证数据展示

  • FC experiment of BALB/C mouse splenocytes using CL488-65058

    FC experiment of BALB/C mouse splenocytes using CL488-65058

    1X10^6 BALB/C mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD31 (CL488-65058, Clone:390), or 0.5 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3). Cells were not fixed.

  • IF Staining of bEnd.3 using CL488-65058

    IF Staining of bEnd.3 using CL488-65058

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CoraLite® Plus 488 CD31 antibody (CL488-65058, Clone: 390 ) at dilution of 1:100.

CoraLite® Plus 488 Anti-Mouse CD31 (390)

CD31 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65058

产品说明书

CloneNo. 390

宿主/亚型

Rat / IgG2a, kappa

种属反应性

Mouse

应用

IF/ICC, FC

别名

C85791, Cd31, Pecam, PECAM 1, Pecam1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inbEnd.3 cells
Positive FC detected inBALB/C mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65058-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65058 targets CD31 in IF/ICC, FC applications and shows reactivity with Mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 Mouse
免疫原 C3H/HeJ mouse hematopoietic progenitor cell line 32D 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule 1
别名 C85791, Cd31, Pecam, PECAM 1, Pecam1
GenBank蛋白编号BC008519
基因名称 CD31
Gene ID (NCBI) 18613
RRIDAB_2883154
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDQ08481
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD31 antibody CL488-65058Download protocol
FC protocol for CL Plus 488 CD31 antibody CL488-65058Download protocol
Standard Protocols
Click here to view our Standard Protocols