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验证数据展示

  • FC experiment of human PBMCs using PE-FcA98124

    FC experiment of human PBMCs using PE-FcA98124

    1x10^6 PHA-treated human PBMCs were surface stained with 5 ul PE Anti-Human CD30/TNFRSF8 Rabbit RecAb (PE-FcA98124, Clone: 241330A12) (red) or 5 ul PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human CD30/TNFRSF8 Rabbit RecAb (PE-FcA98124, Clone: 241330A12) (black, dashed). Cells were not fixed.

FcZero-rAb™ PE Anti-Human CD30/TNFRSF8 Rabbit Recombinant Antibody

CD30/TNFRSF8 FcZero-rAbTM Recombinant Antibody for FC
Cat No. PE-FcA98124

产品说明书

CloneNo. 241330A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CD30, 241330A12, Ki 1 antigen, Ki 1, D1S166E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格: 

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经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-FcA98124 targets CD30/TNFRSF8 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 8
别名 CD30, 241330A12, Ki 1 antigen, Ki 1, D1S166E
计算分子量 64 kDa
GenBank蛋白编号NM_001243
基因名称 CD30
Gene ID (NCBI) 943
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).

实验方案

Product Specific Protocols
FC protocol for PE CD30/TNFRSF8 antibody PE-FcA98124Download protocol
Standard Protocols
Click here to view our Standard Protocols