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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

    FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD19, and 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone:OKT3) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65569-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

    FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone: OKT3) (red) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65569-1-PBS in a different storage buffer formulation.

Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody, PBS Only

CD3 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65569-1-PBS

产品说明书

CloneNo. OKT3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

产品信息

65569-1-PBS targets CD3 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human T cells 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD3e molecule, epsilon (CD3-TCR complex)
别名 CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3
计算分子量 207 aa, 23 kDa
GenBank蛋白编号BC049847
基因名称 CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).