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验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using 65060-1-Ig

    FC experiment of C57BL/6 mouse splenocytes using 65060-1-Ig

    1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug Anti-Mouse CD3ε (65060-1-Ig, Clone:145-2C11) or 0.5 ug Armenian Hamster IgG Isotype Control, and FITC anti-Armenian Hamster IgG antibody at dilution 1:100. Cells were not fixed.

  • IF Staining of mouse splenocytes using 65060-1-Ig

    IF Staining of mouse splenocytes using 65060-1-Ig

    Immunofluorescent analysis of mouse splenocytes using CD3ε antibody (65060-1-Ig, Clone: 145-2C11 ) at dilution of 1:500 and FITC Goat anti-Hamster(Armenian) IgG.

Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for IF/ICC, FC
Cat No. 65060-1-Ig

产品说明书

CloneNo. 145-2C11

宿主/亚型

Hamster / IgG

种属反应性

mouse

应用

IF/ICC, FC

别名

Cd3e, T3e, CD3epsilon, CD3, AI504783

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inC57BL/6 mouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.5 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65060-1-Ig targets CD3ε in IF/ICC, FC applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 mouse
免疫原 H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 种属同源性预测
宿主/亚型 Hamster / IgG
抗体类别 Monoclonal
产品类型 Antibody
全称 CD3 antigen, epsilon polypeptide
别名 Cd3e, T3e, CD3epsilon, CD3, AI504783
GenBank蛋白编号BC098236
基因名称 CD3ε
Gene ID (NCBI) 12501
RRIDAB_2918368
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP22646
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
IF protocol for CD3ε antibody 65060-1-IgDownload protocol
FC protocol for CD3ε antibody 65060-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols