CD28 Polyclonal antibody

CD28 Polyclonal Antibody for IP, IHC, ELISA
Cat No. 26174-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IP, IHC, ELISA

CD28, CD28 molecule, Tp44

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26174-1-AP targets CD28 in IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human
免疫原 CD28 fusion protein Ag24058 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD28 molecule
别名 CD28, CD28 molecule, Tp44
计算分子量 220 aa, 25 kDa
观测分子量44 kDa
GenBank蛋白编号BC093698
基因名称 CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
RRIDAB_3669525
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10747
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

实验方案

Product Specific Protocols
IHC protocol for CD28 antibody 26174-1-APDownload protocol
IP protocol for CD28 antibody 26174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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